Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. 5 Oxoprolinuria as a Cause for Metabolic Acidosis Renal Fellow Network Can Glutathione Tablets Be Your Secret Weapon for Skin Lightening?, Expert Guide Punh: Nutrition Paracetamol toxicity Biochemical Basis Lecture TYLENOL [Acetaminophen] metabolism A lot of GLUTATHIONE is needed to deal with the Oxidative Stress generated by the CYP 450 metabolism of Tylenol. When people go to the ER for Tylenol Overdose