Association between acetaminophen metabolites and CYP2E1 DNA methylation level in neonate cord blood in the Boston Birth Cohort Clinical Epigenetics Springer Nature Link Acetaminophen metabolism Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Can I give Tylenol (acetaminophen) to a patient with elevated aspartate aminotransferase (AST) alanine aminotransferase (ALT) levels?