CNBr Activated Sepharose 4B Cytiva 17 0430 01, pack of 15 g Sigma Aldrich AA467197 controls the hyperactivation of the NLRP3 inflammasome during infection Purification Using GST HiTrap 1 mL and 5 mL Columns: GSTrap 4B, GSTrap FF, and GSTrap HP Protein Phosphatase 4 Is Required for Centrobin Function in DNA Damage Repair Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns: STAR Protocols