Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. What Is Acetaminophen? Definition, Uses, Side Effects, and Risks Olympic Behavioral Health Glutathione, Tylenol & Your Health: What You Should Know The Use and Potential Benefits of N Acetylcysteine in Non Acetaminophen Acute Liver Failure: An Etiology Based Review Frontiers Herbal Therapy for the Treatment of Acetaminophen Associated Liver Injury: Recent Advances and Future Perspectives