Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model Theoretical Biology and Medical Modelling Springer Nature Link Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Why you should avoid REGULAR acetaminophen use for babies and pregnancy 3 Little Plums