Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing) Acetaminophen causes neurodevelopmental injury in susceptible babies and children: no valid rationale for controversy Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA