Acetaminophen cytotoxicity in HepG2 cells is associated with a decoupling of glycolysis from the TCA cycle, loss of NADPH production, and suppression of anabolism Archives of Toxicology Springer Nature Link Don't Suppress a Fever Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Tylenol (acetaminophen) may seem harmless But it could be working against your body's ability to heal. Depletes glutathione, your body's master antioxidant (Prescott, J Clin Invest, 1982) Glutathione is