Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Taking Tylenol? The Glutathione Connection Dr. Green Mom Tylenol depletes something called glutathione. Glutathione is a master antioxidant and your first line of protection against environmental toxins. It detoxifies and protects your cells. It protects your immune system and Tylenol and Glutathione: A Closer Look At Their Relationship Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine